Translational_Unit

Part:BBa_K4855004:Design

Designed by: Slavil Peykov   Group: iGEM23_Bulgaria   (2023-10-11)


J23101 - T7 - medium RBS - amilGFP CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to be cloned in low copy number vectors and used with the dual gene-specific mutator system from Addgene (doi: 10.1093/nar/gkad266)


Source

Assembled following the rules of the BioBrick RFC10 assembly procedure from parts J23101, BBa_K525998, and BBa_K592010. Dual promoters: J23101 for screening and T7 for mutagenesis.

References